Saleh Tabatabaei Vakili; Morteza Mamouei
Volume 18, Issue 2 , June 2016, , Pages 397-408
Abstract
In this Research, effect of bovine serum albumin (BSA) on motility and fertility of indigenous rooster spermatozoa was evaluated using 20 and 48 indigenous roosters and hens, respectively. Semen was collected and pooled. In the first experiment, the pooled semen was diluted and divided into four aliquots.The ...
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In this Research, effect of bovine serum albumin (BSA) on motility and fertility of indigenous rooster spermatozoa was evaluated using 20 and 48 indigenous roosters and hens, respectively. Semen was collected and pooled. In the first experiment, the pooled semen was diluted and divided into four aliquots.The treatments were included the diluted semen without BSA and with 1% BSA; In each group, spermatozoa motility was evaluated during 30 minutes of semen collection and 24 hours after semen storage in 4°C. Spermatozoa motility parameters were evaluated by computer assisted sperm analysis and routine methods. In the second experiment, hens were divided into 4 groups with 12 hens in each group and fresh diluted semen without BSA and containing 1% BSA as well as stored diluted semen without BSA and containing 1% BSA were inseminated. The eggs were incubated and proportion of fertile egg in each treatment group was recorded. By adding 1% BSA into the diluted semen of indigenous rooster, sperm motility parameters and fertility rate were not affected in fresh semen. However, after 24 hours of semen storage in liquid condition, BSA significantly preserved motility and fertility potential of spermatozoa (P<0.05). In conclusion, BSA improved the spermatozoa motility and fertility of indigenous rooster in liquid storage for 24 hours.